Cell Line Description

DerivationPrimary cell type: Mesenchymal stem cell of the bone marrowPrimary cell developmental stage: AdultLocation of primary tissue procurement: Aachen, GermanyTissue collection date: April 21, 2010Passage number reprogrammed: P4Reprogramming methodVector type: IntegratingVector: VirusVirus type: RetrovirusGene list: KLF4MYCPOU5F1SOX2Have the reprogramming vectors been silenced: YesMerthods used: rtpcrNotes on reprogramming vector silencing: Retrovirally encoded reprogramming factors OSKM are not expressed in iPSCs as determined by RT-PCRFiles showing reprogramming vector expressed or silenced: Image / ResultVector map: FileCharacterizationAnalysis of Undifferentiated CellsMarker expression: SSEA-4(+)ZFP42 (TRA1-80(+)TRA 1-80(+)POU5F1 (OCT-4)(+)NANOG(+) Microbiology / Virus ScreeningHIV 1: NegativeHIV 2: Not doneHepatitis B: NegativeHepatitis C: NegativeMycoplasma: NegativeSterilityInoculation for microbiological growth: No Contaminants DetectedMycoplasma: Not DetectedViability: Viable post-cryopreservationGenotypingKaryotypingCell line karyotype: Deletion of the short arm and duplikation of the long arm of Chr20 were detected.Karyotyping method: Molecular karyotyping by SNP arrayGenotypingGenome-wide analysis: Methylation profilingSTR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Cell Line Origin

DepositorKlinikum der Universität zu Köln

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer’;s Disease, Parkinson’;s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington’;s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Legal Information

EBiSC is a trademark of Fraunhofer-Gesellschaft

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation

Linkage

Note: EAUA and CLIP must be completed before order fulfillment

Additional Cell Line Info EBiSC Access Use Agreement (EAUA) Cell Line Information Pack (CLIP)

Subculture Routine

Medium: Essential E8^®Passage method: EDTAMatrix: VitronectinCO₂ concentration: 5%O₂ concentration: 21%Temperature: 37°C