Analysis Note

Single spot by TLC

Biochem/physiol Actions

Primary TargetFas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells

Reversible: no

Product does not compete with ATP.

Cell permeable: yes

General description

A cell-permeable, irreversible, pan-caspase inhibitor. Inhibits Fas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells. Shown to enhance the freeze-thaw survival of human embryonic stem cells. When using with purified native or recombinant enzyme, pre-treatment with an esterase is required.

A cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Heng, B.C., et al. 2007. Biosci. Rep.27, 257.Martin, U., et al. 2007. Apoptosis12, 525.Misaghi, S., et al. 2004. Chem. Biol.11, 1677.Joo, C., et al. 1999. Ophthalmic Res. 31, 287.McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229.Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735.An, S. and Know, K.A. 1996. FEBS Lett.386, 115.Cain, K., et al. 1996. Biochem J.314, 27.Mirzoeva, O.K., et al. 1996. FEBS Lett.396, 266.Pronk, G.J., et al. 1996. Science 271, 808.Slee, E.A., et al. 1996. Biochem. J.315, 21.Chow, S.C., et al. 1995. FEBS Lett. 364, 134.Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283.Zhu, H., et al. 1995. FEBS Lett.374, 303.

Packaging

1, 5 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.

Sequence

Z-Val-Ala-Asp(OMe)-CH₂F*

Warning

Toxicity: Standard Handling (A)