TIRAP Inhibitor Peptide; Cell-Permeable

Biochem/physiol Actions

Reversible: no

Product does not compete with ATP.

Cell permeable: yes

Primary TargetLPS-induced NF-κB activation, PKR phosphorylation, and JNK phosphorylation in RAW.kB cells

General description

A synthetic, cell-permeable peptide that corresponds to mouse toll-interleukin 1 receptor (TIR) domain-containing adapter protein 138-151 (TIRAP; also called Mal (MyD88-adapter-like), fused to the Drosophila antennapedia sequence. Shown to specifically inhibit LPS-induced, but not CpG-induced, NF-κB activation, PKR phosphorylation, and JNK phosphorylation in RAW.κB cells at ~40 µM. Also reported to block IκBα degradation.

A cell-permeable synthetic peptide containing mouse TIRAP_138-151 fused to the Drosophila Antennapedia sequence. Specifically inhibits LPS-, but not CpG-induced NF-κB activation, PKR phosphorylation, and JNK phosphorylation in RAW.κB cells at ~40 µM. Also reported to block IκBα degradation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Toshchakov, V., et al. 2002. Nat. Immunol.3, 392.Fitzgerald, K.A., et al. 2001. Nature413, 78.Henneke, P. and Golenbock, D.T. 2001. Nat. Immunol.2, 828.Horng, T., et al. 2001. Nat. Immunol.2, 835.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

H-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Leu-Gln-Leu-Arg-Asp-Ala-Ala-Pro-Gly-Gly-Ala-Ile-Val-Ser-OH

Warning

Toxicity: Standard Handling (A)