D;L-Sulforaphane

Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetPotent inducer of phase II detoxifying enzymes in mouse tissues and murine hepatoma cells in culture

General description

An isothiocyanate isolated from broccoli that acts as a potent inducer of phase II detoxifying enzymes in mouse tissues and murine hepatoma cells in culture. Shown to be an effective agent in prevention of chemically-induce mammary tumors in rats. Also shown to inhibit the phase I cytochrome P450 isoenzymes 2E1 and IA2 that have been associated with the activation of carcinogens. The induction of phase II enzymes is mediated by the mitogen-activated protein kinase (MAPK) pathway.

An isothiocyanate isolated from broccoli that acts as a potent inducer of phase II detoxifying enzymes in mouse tissues and murine hepatoma cells in culture. It has been shown to be an effective agent in prevention of chemically-induced mammary tumors in rats. It also inhibits the phase I cytochrome P450 isoenzymes 2E1 and IA2 which have been associated with the activation of carcinogens. The induction of phase II enzymes is mediated by mitogen-activated protein kinase (MAPK) pathway.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Gamet-Payrastre, L., et al. 2000. Cancer Res.60, 1426.Yu, R., et al. 2000. J. Biol. Chem.275, 2322.Yu, R., et al. 1999. J. Biol. Chem.274, 27545.Barcelo, S., et al. 1998. Mutat. Res.402, 111.Maheo, K., et al. 1997. Cancer Res.57, 3649.Zhang, Y., et al. 1994. Proc. Natl. Acad. Sci. USA91, 3147.Zhang, Y., et al. 1992. Proc. Natl. Acad. Sci. USA89, 2399.

Packaging

Packaged under inert gas

25 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze DMSO stock solutions (-20°C) and ethanol stock solutions (-70°C). DMSO stock solutions are stable for up to 3 months at -20°C and ethanol stock solutions are stable for up to 3 months at -70°C.

Warning

Toxicity: Standard Handling (A)