InSolution Smoothened Agonist; SAG

General description

A cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (K_D = 59 nM). Shown to induce Hedgehog pathway activation (EC₅₀ ~3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine-KAAD (Cat. No. 239804) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Meloni, A.R., et al. 2006. Mol. Cell. Biol.26, 7750.Chen, W., et al. 2004. Science306, 2257.Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA99, 14071.Frank-Kamenetsky, M., et al. 2002. J. Biol.1, 10.

Packaging

Packaged under inert gas

500 µg in Plastic ampoule

Physical form

A 10 mM (500 µg/86 µl) solution of Smoothened Agonist, SAG (Cat. No. 566660) in H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)