Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetMembrane impermeable DNA intercalator

General description

Extinction coefficient(493 nm): 5900 M ^-1cm^-1;(287 nm): 55,700 M ^-1cm^-1;

Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M^-1cm^-1 (493 nm); 55,700 M^-1cm^-1 (287 nm); 13,800 M^-1cm^-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.

Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Belloc, F., et al. 1994. Cytometry 17, 59.Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.Pollice, A.A., et al. 1992. Cytometry13, 432.

Packaging

1 g in Plastic ampoule

50, 100 mg in Glass bottle

250 mg in Plastic ampoule

Preparation Note

Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Irritant (B)