Biochem/physiol Actions

Cell permeable: no

Primary Targetp56^lck

Reversible: yes

Product competes with ATP.

Target IC₅₀: 4 nM, 5 nM, 5 nM, 100 nM, against p56lck, p59fynT, Hck , and Src, respectively; 600 nM against anti-CD3-stimulated tyrosine phosphorylation of human T cells

General description

A potent and selective inhibitor of the src family of tyrosine kinases. Its action is similar to PP1. Selectively inhibits p56^lck (IC₅₀ = 4 nM), p59^fynT (IC₅₀ = 5 nM), and Hck (IC₅₀ = 5 nM) compared to other tyrosine kinases, such as EGF-R (IC₅₀ = 480 nM), JAK2 (IC₅₀ >50 µM) or ZAP-70 (IC₅₀ >100 µM). Also potently inhibits anti-CD3-stimulated tyrosine phosphorylation of human T cells (IC₅₀ = 600 nM).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hanke, J.H., et al. 1996. J. Biol. Chem.271, 695.Selected CitationsLee, J., et al. 2009. Cell Stem Cell5, 76.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Physical form

A 10 mM (1 mg/331 µl) solution of PP2 (Cat. No. 529573) in DMSO.

Reconstitution

Following initial thaw, aliquot, purge with inert gas, and freeze (-20°C).

Warning

Toxicity: Irritant (B)