Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary Targetserine proteases

General description

Irreversible inhibitor of serine proteases. Its mechanism of action is analogous to that of diisopropylfluorophosphate. PMSF causes sulfonylation of the active-site serine residues. Also reported to inhibit internucleosomal DNA fragmentation in immature thymocytes. Effective at a concentration of 50 µM.

Irreversible inhibitor of serine proteases. Its mechanism of action is analogous to that of diisopropylfluorophosphate. PMSF causes sulfonylation of the active-site serine residues. Also reported to inhibit internucleosomal DNA fragmentation in immature thymocytes. For a related, more stable inhibitor, see AEBSF (Cat. No. 101500).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Seitz, R., et al. 1993. Int. J. Cancer53, 514.Weaver, V.M., et al. 1993. Biochem. Cell. Biol.71, 488.Bourgain, R.H., et al. 1992. Adv. Exp. Med. Biol.316, 427.Chang, C.T., et al. 1992. Biochem. Int.28, 707.

Packaging

5 g in Glass bottle

1 g in Alu drum

25 g in Fibre case

Reconstitution

t_½ = 1 hour at pH 7.5. Effective concentration: 50 µM.

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 9 months at 4°C.

Warning

Toxicity: Corrosive (L)