Pepstatin A; Penetratin

General description

A Pepstatin A (PepA; Cat. No. 516481) penetratin (pAntp_43-58) conjugate (PepA-P) that is ~75% less potent against Cathespins D/E (IC₅₀ = 920 nM) than PepA in cell-free assays, presumably due to its decreased solubility. However, with its enhanced permeability, PepA-P is much more active than PepA in inhibiting cellular aspartic protease activity in MCF7 (94.1% vs. 54.6% inhibition wtih 10 µM respective compound), Boleths, and Dendritic cultures. PepA-P, but not PepA, effectively interferes with antigen processing by primary APCs and the subsequent activation of antigen-specific memory T cells.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Zaidi, N., et al. 2007. Biochem. Biophys. Res. Commun.364, 243.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

PepA-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-CO₂H

Warning

Toxicity: Standard Handling (A)