p75NTR Signaling Inhibitor; Cell-permeable; TAT-Pep5

Biochem/physiol Actions

Reversible: no

Primary TargetBlocker of MAG- and Nogo-induced inhibition of neurite outgrowth

Product does not compete with ATP.

Cell permeable: yes

General description

The p75^NTR inhibitor Pep5 (Cat. No. 506180) fused to the N-terminal, 11-amino acid protein transduction domain sequence from the HIV TAT protein for membrane permeability. Show to inhibit the association of p75^NTR with Rho-GDI in vitro. Reported to act as an effective blocker of MAG- and Nogo-induced inhibition of neurite outgrowth in dorsal root ganglion (DRG) and postnatal cerebellar neurons at a concentration of 100 nM. Reported to be a potential therapeutic agent for the inhibition of regeneration in the central nervous system.

The p75^NTR inhibitor Pep5 (Cat. No. 506180) is made cell-permeable by fusing it with the N-terminal protein transduction domain sequence (11 amino acids) from HIV protein TAT. Shown to act as an effective blocker of MAG- and Nogo-induced inhibition of neurite outgrowth in both DRG (dorsal root ganglion) and postnatal cerebellar neurons. A potential therapeutic agent against inhibition of regeneration in the central nervous system.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Yamashita, T., and Tohyama, M. 2003. Nat. Neurosci.6, 461.Schwarze, S.R., et al. 1999. Science285, 1569.

Packaging

Packaged under inert gas

500 µg in Plastic ampoule

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Sequence

H₂N-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Cys¹²-Phe-Phe-Arg-Gly-Gly-Phe-Phe-Asn-His-Asn-Pro-Arg-Tyr-Cys²⁶-OHr>disulfide bond (12-26)

Warning

Toxicity: Standard Handling (A)