InSolution MIF Antagonist; ISO-1

Biochem/physiol Actions

Primary TargetMIF dopachrome tautomerase

Cell permeable: yes

General description

A cell-permeable isoxazoline compound that displays anti-inflammatory properties. Inhibits MIF dopachrome tautomerase activity by binding to its catalytic active site (IC₅₀ = 7 µM for D-dopachrome tautomerase) and suppresses the production of TNFα, PGE₂ and COX-2 in human monocytes and arachidonic acid in RAW 264.7 macrophages. Shown to exhibit antidiabetogenic properties in immunoinflammatory diabetic mouse model.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Cvetkovic, I., et al. 2005. Endocrinology146, 2942.Lubetsky, J.B., et al. 2002. J. Biol. Chem.277, 24976.

Packaging

Packaged under inert gas

10 mg in Glass bottle

Physical form

A 50 mM (10 mg/850 µL) sterile-filtered solution of MIF Antagonist, ISO-1 (Cat. No. 475837) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.

Warning

Toxicity: Irritant (B)