InSolution Brefeldin A; Eupenicillium brefeldi

Biochem/physiol Actions

Reversible: yes

Primary TargetADP-ribosylation factor (ARF)

Cell permeable: yes

General description

Specifically and reversibly blocks translocation of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus without affecting endocytosis or lysosome function. Inhibitor of HDL-mediated cholesterol efflux. Causes disassembly of the Golgi complex and ER swelling in a variety of mammalian cell lines at ﹤40 ng/ml. Inhibits the activity of BIG1 and BIG2, the guanine-nucleotide exchange proteins for ADP-ribosylation factors. Blocks binding of ADP-ribosylation factor to the Golgi and inhibits the GDP-GTP exchange.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Yamagi, R., et al. 2000. Proc. Natl. Acad. Sci. USA97, 2567.Moriango, N., et al. 1996. Proc. Natl. Acad. Sci. USA93, 12856.Mendez, A.J. 1995. J. Biol. Chem.270, 5891.Ridgway, N.D., et al. 1995. J. Biol. Chem. 270, 8023.Tsai, S.C., et al. 1994. Proc. Natl. Acad. Sci. USA91, 3063.Ktistakis, N.Y., et al. 1992. Nature356, 344.

Packaging

Packaged under inert gas

2 mg in Glass bottle

Physical form

A 25 mM (2 mg/285 µL) solution of (+)-Brefeldin A (Cat. No. 203729) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C. Use only fresh DMSO for further dilutions prior to use.

Warning

Toxicity: Irritant (B)