Amino Acid Sequence

Z-Leu-Leu-Leu-al

General description

Potent, reversible, and cell-permeable proteasome inhibitor (K_i = 4 nM). Reduces the degradation of ubiquitin-conjugated proteins in mammalian cells and permeable stains of yeast by the 26S complex without affecting its ATPase or isopeptidase activities. Activates c-Jun N-terminal kinase (JNK1) which initiates apoptosis. Inhibits NF-κB activation (IC₅₀ = 3 µM). This material is ≥95% pure by HPLC. A material with ≥98% purity by HPLC (Cat. No. 474790) is also available. Also available as a 20 mM solution in DMSO (Cat. No. 474788).

Potent, reversible, and cell-permeable proteasome inhibitor (K_i = 4 nM). Reduces the degradation of ubiquitin-conjugated proteins in mammalian cells and permeable stains of yeast by the 26S complex without affecting its ATPase or isopeptidase activities. Activates c-Jun N-terminal kinase (JNK1) which initiates apoptosis. Inhibits NF-κB activation (IC₅₀ = 3 µM). This material is ≥95% pure by HPLC. A material with ≥98% purity by HPLC (Cat. No. 474790) is also available.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Steinhilb, M.L., et al. 2001. J. Biol. Chem.276, 4476.Meriin, A.B., et al. 1998. J. Biol. Chem. 273, 6373.Adams, J., and Stein, R. 1996. Ann. Rep. Med. Chem.31, 279.Klafki, H.W., et al. 1996. J. Biol. Chem.271, 2865.Lee, D.H., and Goldberg, A.L., 1996. J. Biol. Chem.271, 27280.Wiertz, E.J., et al. 1996. Cell84, 769.Jensen, T.J., et al. 1995. Cell 83, 129.Read, M.A., et al. 1995. Immunity2, 493.Rock, K.L., et al. 1994. Cell 78, 761.

Packaging

Packaged under inert gas

10 mg in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)