Analysis Note

Positive ControlCat, dog, human, or monkey mast cells

Application

Frozen Sections (0.06-0.12 µg/ml)r>Immunoblotting (1 µg/ml)r>Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)

General description

This Anti-Mast Cell Tryptase Mouse mAb (AA1) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of Mast Cell Tryptase.

Protein G purified mouse monoclonal antibody. Recognizes the ~32-38 kDa α- and β-isoforms of tryptase in mast cells.

Recognizes the 32-38 kDa α- and β-isoforms of tryptase in mast cells.

Immunogen

native, human mast cell, tryptase

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Staining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.

Sommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.Little, S.S., and Johnson, D.A. 1995. Biochem. J.307, 341.Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol.87, 111.Walls, A.F., et al. 1990. J. Pathol.162, 119.Wall, A.F., et al. 1990. Clin. Exp. Allergy20, 581.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)