Biochem/physiol Actions

β-Lactamasehydrolyzesβ-lactumantibioticsandisthechief cause ofresistance to β-lactam antibiotics development by bacteria. Mutationsin theβ-lactamases are associated with extended-spectrum β-lactamases (ESBLs).

General description

β-Lactamaseproduced bybacteriashares sequence homology topenicillin-binding proteins. This enzyme is found inGram-negativebacteria. β-Lactamaseshave four molecularclasses, named A, B, C, and D. A, C, and D form an acyl-enzyme viaactive site serine residue.Class B β-lactamasesaremetalloenzymes, which have a zinc ion attheiractive site for β-lactam hydrolysis.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

500 u in Glass bottle

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 month at 4°C.

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol benzyl penicillin and 1.0 µmol of cephalosporin C, respectively, per min at 25°C.

Warning

Toxicity: Harmful (C)