Biochem/physiol Actions

Cell permeable: no

Reversible: no

Shown to undergo phosphorylation by JNK in the presence of ³²P-ATP.

Primary TargetHighly specific substrate for c-Jun N-terminal kinase/stress activated protein kinase (JNK/SAPK)

Product does not compete with ATP.

General description

Recombinant c-Jun (1-79) activation domain fused to GST and expressed in E. coli. Highly specific substrate for c-Jun N-terminal kinase/stress activated protein kinase (JNK/SAPK). Is not phosphorylated by p42 MAP kinase or p38. Suitable for qualitative and quantitative assays of JNK activity. Shown to undergo phosphorylation by JNK in the presence of ³²P-ATP.

GST fusion protein of c-jun (1-79) activation domain, expressed in E. coli. Highly specific substrate form c-jun N-terminal kinase/stress activated protein kinase (JNK/SAPK). Not phosphorylated by MAP kinase or p38. Suitable for qualitative and quantitative assays of JNK activity.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Derijard, B., et al. 1994. Cell 76, 1025.Kyriakis, J.M., et al. 1994. Nature369, 156.Hibi, M., et al. 1993. Genes Dev.7, 2135.

Packaging

100 µg in Plastic ampoule

Physical form

In 50 mM NaCl, 20 mM Tris-HCl, 2 mM EDTA, 1 mM DTT, 50% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)