Insulin Degrading Enzyme; Hi 1PC X 50UG

General description

Recombinant, rat insulin degrading enzyme fused to a His Tag™ sequence and expressed in S. frugiperda insect cells. A metalloprotease that degrades insulin and a variety of other peptides including amyloid peptides.

Recombinant, rat insulin degrading enzyme fused to a His Tag™ sequence and expressed in S. frugiperda insect cells. Insulin degrading enzyme (Insulysin) is a metalloprotease that degrades insulin and a variety of other peptides, including glucagon, insulin-like growth factors I and II, atrial natriuretic peptide, TGF-α, and amyloid peptides. Reported to be inversely correlated with brain Aβ peptide levels in insulysin-deficient mice compared to wild-type mice.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hersh, L.B. 2003. Curr. Pharm. Des.9, 449.Miller, B.C., et al. 2003. Proc. Natl. Acad. Sci. USA100, 6221.Song, E.S., et al. 2003. J. Biol. Chem.278, 49789.Mukherjee, A., and Hersh, L.B. 2002. J. Alzheimer’;s Dis.4, 341.Song, E.S., et al. 2001. J. Biol. Chem.276, 1152.Becker, A.B., and Roth, R.A. 1995. Methods Enzymol.248, 693.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM potassium phosphate buffer, 20% glycerol, pH 7.3.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of the enzyme that will catalyze the hydrolysis of 1 µmol iodinated insulin per h at 37°C, pH 7.3.

Warning

Toxicity: Standard Handling (A)