Caspase-1 Inhibitor I; Cell-Permeable

Biochem/physiol Actions

Reversible: yes

Product does not compete with ATP.

Primary TargetCaspase-1

Cell permeable: yes

General description

A cell-permeable inhibitor of caspase-1 (ICE; interleukin-1β converting enzyme). The C-terminal YVAD-CHO sequence of this peptide is a highly specific, potent, and reversible inhibitor of caspase-1 (K_i = 1 nM). The N-terminal sequence (residues 1-16) corresponds to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF) and confers cell-permeability to the peptide.

A cell-permeable inhibitor of caspase-1 (ICE; Interleukin-1β Converting Enzyme), caspase-4, and caspase-5. The C-terminal YVAD-CHO sequence of this peptide is a highly specific, potent, and reversible inhibitor of caspase-1 (K_i = 1 nM). The N-terminal sequence (amino acid residues 1-16) corresponds to the hydrophobic region (h-region) of the signal peptide of the Kaposi fibroblast growth factor (K-FGF) and confers cell-permeability to the peptide.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Garcia-Calvo, M., et al. 1998. J. Biol. Chem. 273, 32608.Hilbi, H., et al. 1998. J. Biol. Chem. 273, 32895.Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.Lin, Y.-Z., et al. 1996. J. Biol. Chem.271, 5305.Lin, Y.-Z., et al. 1995. J. Biol. Chem.270, 14255.Reiter, L.A. 1994. Int. J. Pept. Protein Res.43, 87.Thornberry, N.A., et al. 1994. Biochemistry33, 3934.Thornberry, N.A., et al. 1994. MethodsEnzymol. 244, 615.Thornberry, N.A., et al. 1992. Nature 356, 768.

Packaging

1 mg in Plastic ampoule

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Sequence

Ac-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Tyr-Val-Ala-Asp-CHO

Warning

Toxicity: Standard Handling (A)