Caspase-1 Inhibitor I

Biochem/physiol Actions

Primary Targetcaspase-1, human recombinant

Cell permeable: no

Target K_i: 200 pM for human recombinant caspase-1

Reversible: yes

Product does not compete with ATP.

General description

A potent, specific, and reversible inhibitor of caspase-1 (K_i = 200 pM for human recombinant caspase-1), caspase-4, and caspase-5. Strongly inhibits anti-APO-1 induced apoptosis in L929-APO-1 cells.

Interleukin-1β converting enzyme (ICE) is a cysteine protease that cleaves the IL-1β precursor to mature IL-1β. ICE inhibitor I is a potent, specific, and reversible inhibitor of ICE (K_i = 200 pM for human recombinant ICE). Strongly inhibits anti-APO-1 induced apoptosis in L929-APO-1 cells.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Garcia-Calvo, M., et al. 1998. J. Biol. Chem. 273, 32608.Hilbi, H., et al. 1998. J. Biol. Chem. 273, 32895.Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.Los, M., et al. 1995. Nature375, 81.Walker, N.P.C., et al. 1994. Cell78, 343.Wilson, K.P., et al. 1994. Nature370, 270.Thornberry, N.A., et al. 1992. Nature356, 768.

Packaging

1, 5 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Sequence

Ac-Tyr-Val-Ala-Asp-CHO

Warning

Toxicity: Standard Handling (A)