General description

Recombinant HIV reverse transcriptase expressed in E. coli. Catalyzes error-prone synthesis from DNA and RNA templates. Provides an excellent target for evaluating antiviral agents.

Recombinant HIV reverse transcriptase expressed in E. coli. Catalyzes error-prone synthesis on DNA and RNA templates. Provides an excellent target for evaluating antiviral agents.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

500 u in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM potassium phosphate, 1 mM DTT, 20% glycerol, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 1.0 nmol of labeled dTTP into acid-insoluble material in 20 min at 37°C, pH 8.3.

Warning

Toxicity: Standard Handling (A)