Bisbenzimide H 33258 Fluorochrome; Trihydrochloride

Biochem/physiol Actions

Reversible: no

Primary TargetAdenine-thymine-specific fluorescent stain

Product does not compete with ATP.

Cell permeable: yes

General description

Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca²⁺ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.

Membrane-permeant, adenine-thymidine specific fluorescent stain (excitation maximum: 346 nm; emission maximum: 460 nm). Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca²⁺ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Werstuck, G., and Green, M.R. 1998. Science 282, 296.Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.Tarshis, M., et al. 1994. J. Immunol. Methods168, 245.Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.Oberhammer, F., et al. 1993. EMBO J. 12, 3679.Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods162, 41.Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA85, 8939.Cesarone, C.F., et al. 1979. Anal. Biochem.100, 188.Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem.24, 24.

Packaging

1 g in Plastic ampoule

100, 250 mg in Plastic ampoule

Preparation Note

Precipitates in phosphate buffered solutions

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C.

Warning

Toxicity: Carcinogenic / Teratogenic (D)