D-erythro-Sphingosine; N-Hexanoyl-

Biochem/physiol Actions

Effective concentration: 10 nM in activating protein phosphatase 2A (PP2A)

Reversible: no

Product does not compete with ATP.

Cell permeable: yes

Primary TargetMAP kinase, protein phosphatase 2A (PP2A)

General description

Biologically active, cell-permeable, non-physiological ceramide analog. Induces a dramatic arrest in the G₀/G₁ phase of the cell cycle. Activates MAP kinase. Activates protein phosphatase 2A (PP2A) at 10 nM. Inhibits diacylglycerol accumulation and phospholipase D (PLD) activation in fibroblasts. Induces apoptosis in MOLT-4 leukemia cells.

Biologically active, cell-permeable, nonphysiological ceramide analog. Stimulates cytosolic serine/threonine protein phosphatase in T9 cells and induces phosphorylation on Thr-669 in A-431 cells by stimulation of ceramide-activated protein kinase. Induces a dramatic arrest in the G_o/G₁ phase of the cell cycle. Activates MAP kinase. Activates protein phosphatase 2A (PP2A) at 10 nM. Inhibits diacylglycerol accumulation and phospholipase D activation in fibroblasts. Induces apoptosis in Molt-4 leukemia cells.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Jayadev, S., et al. 1995. J. Biol. Chem. 270, 2047.Venable, M.E., et al. 1994. J. Biol. Chem. 269, 26040.Dobrowsky, R.T., et al. 1993. J. Biol. Chem. 268, 15523.Raines, M.A., et al. 1993. J. Biol. Chem.268, 14572.Dobrowsky, R.T., and Hannun, Y.A. 1992. J. Biol. Chem.267, 5048.

Packaging

5 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.

Warning

Toxicity: Irritant (B)