General description

A cell-permeable acylurea compound that acts as an antagonistic Smo ligand (IC₅₀ = 120 nM against 5 nM Bodipy-cyclopamine binding to murine Smo-transfected HEK293 cells) and is more effective than Cyclopamine (Cat. No. 239803) in inhibiting ShhN-induced Gli transcription in Shh-light 2 cell assays (IC₅₀ = 0.16 and 0.3 µM, respectively) and in blocking 0.1 µM SAG- (Cat. Nos. 566660 and 566661) stimulated differentiation of C3H10T1/2 murine mesenchymal stem cells into osteoblasts (IC₅₀ = 0.13 and 0.62 µM, respectively).

A cell-permeable acylurea compound that acts as an antagonistic Smo ligand (IC₅₀ = 120 nM against 5 nM Bodipy-cyclopamine binding to murine Smo-transfected HEK293 cells) and is more effective than Cyclopamine (Cat. No. 239803) in inhibiting ShhN-induced Gli transcription in Shh-light 2 cell assays (IC₅₀ = 0.16 and 0.3 µM, respectively) and in blocking 0.1 µM SAG- (Cat. Nos. 566660 & 566661) stimulated differentiation of C3H10T1/2 murine mesenchymal stem cells into osteoblasts (IC₅₀ = 0.13 and 0.62 µM, respectively).

The Hh Signaling Antagonist XI, MRT-14 controls the biological activity of Hh pathway. This small molecule/inhibitor is primarily used for Cell Signaling applications.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Manetti, F., et al. 2010. Mol. Pharmacol.78, 658.

Packaging

Packaged under inert gas

10 mg in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Standard Handling (A)