General description

A cell-permeable thiosemicarbazone copper chelator that potently blocks the HDL receptor SR-BI- (scavenger receptor, class B, type I) mediated lipid uptake (IC₅₀ = 21 and 57 nM against DiI and CE uptake, respectively, from DiI-HDL and CE-HDL using hamster ldlA-7 cells expressing murine SR-BI) in a reversible manner, while exerting little effect toward intracellular membrane trafficking or cytoskeletal organization. BLT-1 chelates Cu⁺² in a 2:1 stoichiometric ratio and a half molar equivalent of CuCl₂ is shown to fully prevent zebrafish embryos from BLT-1-induced development defects, while BLT-1 can likewise be used to chelate excess amount of copper (>4 µM) and rescue embryos from copper toxicity.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Nieland, T.J.F., et al. 2008. Biochemistry47, 460.Raldua, D., and Babin, P.J. 2007. Toxicol. Lett.175, 1.Nieland, T.J.F., et al. 2002. Proc. Natl. Acad. Sci. USA99, 15422.Peterson, R.T., et al. 2000. Proc. Natl. Acad. Sci. USA97, 12965.

Packaging

Packaged under inert gas

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Standard Handling (A)