Components

SimulFluor^® HSV/VZV - (Catalog No. 5235). One 5 mL dropper vial containing monoclonal antibodies specific for HSV antigen and specific for VZV antigen, protein stabilizer, Evans blue and 0.1% sodium azide (preservative).HSV Control Slides - (Catalog No. 5093). Two slides containing one well of HSV -1 infected HEp-2 cells, one well of HSV -2 infected cells, and one well of uninfected cells.VZV Control Slides - (Catalog No. 5088). Two slides containing one well of VZV (clinical isolate) infected human foreskin fibroblasts and one well of uninfected human foreskin fibroblasts.Phosphate-Buffered Saline (PBS) - (Catalog No. 5087). One packet of phosphate buffered saline salts.Tween 20 / Sodium Azide Solution (100X) - (Catalog No. 5037). One 10 mL vial containing Tween 20/sodium azide concentrate.Mounting Fluid - (Catalog No. 5013). One 10 mL dropper vial containing Tris-buffered glycerin, a fluorescence enhancer, and 0.1% sodium azide (preservative).

Disclaimer

For in vitro Diagnostic UseCE Mark

General description

Diagnostics Kit

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

SimulFluor is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage and Stability

When stored at 2-8°C, the SimulFluor^® HSV/VZV Immunofluorescence Assay kit is stable up to the expiration date printed on the kit label. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the kit expiration date.

Warnings and Precautions:

⋅ For In Vitro Diagnostic use

⋅ The sodium azide (NaN3) used as a preservative in the SimulFluor™ reagent, PBS/Tween, and Mounting Fluid is toxic if ingested. Sodium azide may react with lead and copper plumbing to form highly explosive metal azides (15,16). Upon disposal, flush with large volumes of water to prevent build-up in plumbing.

⋅ Pooling or alteration of any reagent may cause erroneous results.

⋅ Do not substitute reagents from other manufacturers.

⋅ Incubation times or temperatures other than those specified may give erroneous results. Any such change must be validated by the user.

⋅ Do not allow shell vials or slides to dry at any time during the staining procedure.

⋅ Handle all specimens and materials, coming in contact with them as potentially infectious and dispose of with proper precautions. Decontaminate with 0.05% sodium hypochlorite.

⋅ Do not mouth pipette reagents.

⋅ Acetone is extremely flammable and harmful if swallowed or inhaled. Keep away from heat, sparks or flame. Avoid breathing vapor. Use adequate ventilation.

⋅ Avoid contact with Evans blue (present in the SimulFluor™ reagent) as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water.

⋅ Mounting Fluid contains a fluorescence enhancer that may be destructive to mucous membranes. Avoid direct skin or mucous membrane contact. If contact occurs, flush with large volumes of water.