4,6-Diamidino-2-phenylindole dihydrochloride, suitable for fluorescence, BioReagent, >=95.0% (HPLC)

Code: 32670-25MG-F D2-231

Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probe...


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€393.00 25MG
€483.39 inc. VAT
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Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

assay≥95.0% (HPLC)
fluorescenceλex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 340 nm; λem 488 nm (nur DAPI), λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA
formpowder
InChI keyFPNZBYLXNYPRLR-UHFFFAOYSA-N
InChI1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
product lineBioReagent
Quality Level100
SMILES stringCl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
solubilityDMF: soluble, H2O: soluble
suitabilitysuitable for fluorescence
Cas Number28718-90-3
This product has met the following criteria to qualify for the following awards:



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