Biochem/physiol Actions

Cell permeable: no

Reversible: no

Primary TargetChromogenic substrate for human neutrophil elastase

Product does not compete with ATP.

Kcat/Km = 120,000 vs. 2700 for pancreatic elastase

General description

Specific chromogenic substrate for human neutrophil elastase (k_cat/K_m = 120,000 versus pancreatic elastase: k_cat/K_m = 2,700). Cleavage can be monitored at 410 nm using ε = 8,800.

A specific chromogenic substrate for human neutrophil elastase (K_cat/K_m = 120,000 vs. 2700 for pancreatic elastase). Cleavage can be monitored at ~410 nm. Sold on the basis of substrate content.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Barrett, A.J., et al. 1989. Biochim. J.260, 259.Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.

Packaging

10 mg in Glass bottle

Physical form

Lyophilized from a solution containing 90 mg D-mannitol and 10 mg substrate.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

MeOSuc-Ala-Ala-Pro-Val-pNA

Warning

Toxicity: Standard Handling (A)