InSolution eIF4E/eIF4G Interaction Inhibitor; 4EGI-1

Biochem/physiol Actions

Reversible: yes

Primary TargeteIF4E

Cell permeable: yes

General description

A cell-permeable hydrazone compound that reversibly binds eukaryotic translation initiation factor 4E (eIF4E; K_D = 25 µM) and disrupts eIF4E/eIF4G, but not eIF4E/4E-BP1, complex formation. Reported to downregulate the protein, but not mRNA, levels of c-Myc and Bcl-x_L in Jurkat cells (8 hrs), leading to apoptotic cell death after prolonged exposure (24 hrs, IC₅₀ ~25 µM). Shown to be brain permeant and reduce neuroligin protein levels, and restore L-LTP threshold and rescue autistic-like behaviours in Eif4ebp2 knockout mice.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Gkogkas, C.G., et al. 2012. Nature493, 371.Moerke, N. J., et al. 2007. Cell128, 257.

Packaging

5 mg in Glass bottle

Packaged under inert gas

Physical form

A 25 mM (5 mg/443 µL) solution of eIF4E/eIF4G Interaction Inhibitor, 4EGI-1 (Cat. No. 324517)

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C.

Warning

Toxicity: Irritant (B)