General description

Note: 1 mU = 1 milliunit.

DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes.

Recombinant, human DPP IV fused at the N-terminus to a His Tag™ sequence and expressed in S. frugiperda insect cells. A serine exopeptidase dimer composed of two identical subunits of 110-130 kDa. DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes. Note: 1 mU = 1 milliunit.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Pospisilik, JA, et al. 2003. Diabetes52, 741.Dobers, J., et al. 2002. Protein Expr. Purif.25, 527.Marguet, D., et al. 2000. Proc. Natl. Acad. Sci.97, 6874.Misumi, Y., et al. 1992. Biochim. Biophys. Acta1131, 333.

Packaging

5 miu in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 20 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmole H-Gly-Pro-AMC per min at 37°C, pH 8.0.

Warning

Toxicity: Standard Handling (A)