Diethyl Pyrocarbonate

Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetInhibits ryanodine binding to ryanodine/Ca2+ receptor channel in skeletal muscle

General description

Inhibits ryanodine binding to ryanodine/Ca²⁺ receptor channel in skeletal muscle. Useful for specific inactivation of nucleases during isolation of undegraded polynucleotides. Also inhibits platelet-activating factor acetyl hydrolase. Has been used as a probe for the topography of 5.8S rRNA in yeast ribosomes.

Inhibits ryanodine binding to ryanodine/Ca²⁺ receptor channel in skeletal muscle in a dose- and time-dependent manner and increases the Ca²⁺ permeability of SR vesicles. Useful for specific inactivation of nucleases during isolation of undegraded polynucleotides. Also inhibits platelet-activating factor acetyl hydrolase. Has been used as a probe for the topography of 5.8S rRNA in yeast ribosomes.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Shoshan-Barmatz, V., and Weil, S. 1994. Biochem. J. 299, 177.Blumbers, D.O. 1987. Methods Enzymol. 152, 20.

Packaging

100 g in Glass bottle

25 g in Plastic ampoule

Warning

Toxicity: Harmful (C)