Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary Targetserine proteases

General description

A potent, irreversible inhibitor of serine proteases. Reacts with serine proteases to release an acylchloride moiety that can acylate another active site residue. Inhibits granzyme B and blocks apoptotic internucleosomal DNA cleavage in thymocytes without the involvement of endonucleases. Does not affect thiol proteases and metalloproteases. Does not exhibit any activity towards β-lactamases.

A potent irreversible inhibitor of serine proteases. Blocks apoptotic internucleosomal DNA cleavage in thymocytes without the involvement of endonucleases. Reacts with serine proteases to release an acylchloride moiety that can acylate another active site residue. Does not affect thiol proteases and metalloproteases. Also has no activity towards β-lactamases. Effective at concentrations ranging from 5-100 µM.

Effective concentration: 5-100 µM; t_½ = 20 min at pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Cain, K., et al. 1994. Biochem. Cell Biol.72, 631.Weaver, V.M., et al. 1993. Biochem. Cell Biol. 71, 488.Odake, S., et al. 1991. Biochemistry 30, 2217.Harper, J.W., et al. 1985. Biochemistry24, 1831.

Packaging

10 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)