Application

A useful alternative to lactoperoxidase for ¹³¹I ion labeling studies, for bromination of proteins, and for ³⁶Cl labeling of macromolecules in long-term isolation procedures.

Biochem/physiol Actions

Chloroperoxidase (CPO) helps in the catalysis of oxidation reactions with the help of hydrogen peroxide. It displays peroxidase, catalaseandcytochromeP450-like functions. CPO also plays a role in catalyzinghalogenationreactions.

General description

Chloroperoxidase (CPO) is a major heme-containing, synthetic and versatile enzyme, obtained fromCaldariomyces fumago.

Other Notes

Oxidation of aminopyrine; Chloroperoxidase, a peroxidase with potential; Chloroperoxidase-catalyzed asymmetric transformations.

Physical form

Supplied as a suspension in 0.1 M sodium phosphate pH 4.0.

Unit Definition

1 U corresponds to the amount of enzyme which converts 1 µmol of monochlorodimedone to dichlorodimedone per minute at pH 2.75 and 25 °C in the presence of KCl and H₂O₂.