Biochem/physiol Actions

Primary TargetNitric oxide (NO) fluorescent indicator

General description

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Manufactured by Daiichi Pure Chemicals Co., Ltd

Other Notes

Kojima, H., et al. 2001. J. Neurochem.76, 1404.Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.Itoh, Y., et al. 2000. Anal. Biochem.287, 203.Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.

Packaging

1 mg in Glass bottle

Physical form

A 5 mM solution in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.

Warning

Toxicity: Irritant (B)