Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetFluorogenic caspase-3 substrate

Km = 9.7 ± 1.0 µM for cleavage of this substrate by caspase-3

General description

Fluorogenic CPP32/Apopain substrate. Cleavage of this substrate by CPP32/Apopain shows Michaelis-Menten kinetics (K_m = 9.7 ± 1.0 µM). Cleavage can be monitored by excitation at 380 nm and emission at 460 nm.

Fluorogenic caspase-3 substrate. Cleavage of this substrate by caspase-3 shows Michaelis-Menten kinetics (K_m = 9.7 ± 1.0 µM). Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hermission, M., et al. 2000. Oncogene 19, 2338.Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.Nicholson, D.W., et al. 1995. Nature 376, 37.

Packaging

1, 5 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

Ac-Asp-Glu-Val-Asp-AMC

Warning

Toxicity: Standard Handling (A)