Cleland#s Reagent 1PC X 25GM

General description

Oxidized dithiothreitol: ≤0.5%.

A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations.

DTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes. Protects protein sulfhydryls and restores enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL™ Reagent, Cat. No. 233157) can be used. Oxidized dithiothreitol: ≤0.5%.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Gailis, L. 1994. Eur. J. Pharmacol. 292, 39.Lauriault, V.V.M., and O′Brien, P.J. 1991. Mol. Pharmacol.40, 125.Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett.42, 199.Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem.243, 716.Cleland, W.W. 1964. Biochemistry3, 480.

Packaging

25, 100, 500 g in Glass bottle

Packaged under inert gas

1, 5, 10 g in Plastic ampoule

1 kg in Glass bottle

Reconstitution

Following reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Harmful (C)