Biochem/physiol Actions

Reversible: no

Primary Targetcathepsin B

Product does not compete with ATP.

k₂/K_i = 8.9 x 10³ M_-¹sec_-¹ for cathepsin B

Cell permeable: yes

General description

Cell-permeable cysteine protease inhibitor. Inhibits cathepsin B (k₂/K_i = 8.9 x 10³ M^-1 sec^-1), cathepsin L (k₂/K_i = 3.8 x 10⁵ M^-1 sec^-1), cathepsin S (k₂/K_i = 4.2 x 10⁴ M^-1 sec^-1), and papain (k₂/K_i = 2.4 x 10³ M^-1 sec^-1).

Cysteine protease inhibitor. Selectively inhibits cathepsin B (k₂/K_i = 8.9 x 10³ M^-1sec^-1), cathepsin L (k₂/K_i = 3.8 x 10⁵ M^-1sec^-1), cathepsin S (k₂/K_i = 4.2 x 10⁴ M^-1sec^-1), and papain (k₂/K_i = 2.4 x 10³ M^-1sec^-1). Half-life in buffered solution (pH 5-9) = 25 h (30°C); 300 h (0°C).

Half-life in buffered solution (pH 5-9) = 25 h (30°C); 300 h (0°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Zhu, D. and Uckun, F.M. 2000. Clin. Cancer Res.6, 2064.Demuth, H.-U., et al. 1996. Biochim. Biophys. Acta 1295, 179.Brömme, D., and Demuth, H.-U. 1994. Methods Enzymol. 244, 671.

Packaging

1 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

Z-Phe-Gly-NHO-Bz

Warning

Toxicity: Standard Handling (A)