#-Bungarotoxin, Bungarus mul 1PC x 1MG

Analysis Note

Contaminants: None detectable by IEF

Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetMotor end-plate acetylcholine receptor

Kd = 1 pM to 1 nM for motor end-plate acetylcholine receptor

General description

Blocks neuromuscular transmission by irreversible binding to motor end-plate acetylcholine receptor (K_d = 1 pM to 1 nM) but does not depress acetylcholine release from motor nerve endings. Blocks nicotine-induced increase of intracellular Ca²⁺ in PC12 cells (IC₅₀ = 310 nM), and prevents opening of nicotinic receptor-associated ion channels. Reconstitution experiments in Xenopus oocytes have shown the effects of α-bungarotoxin on neuronal nAChR to be highly specific for the α₇-subtype (IC₅₀ = 1.6 nM), but not for the α₃β₄-subtype (IC₅₀ >3 µM).

A polypeptide composed of 74 amino acids containing 5 disulfide bridges. Blocks neuromuscular transmission by irreversible binding to the motor end-plate acetylcholine receptor (K_d = 1 nM to 1 pM) but does not depress acetylcholine release from motor nerve endings. Blocks nicotine-induced augmentation in intracellular Ca²⁺ in PC12 cells (IC₅₀ = 310 nM). Prevents opening of nicotinic receptor-associated ion channels. Reconstitution experiments in Xenopus oocytes have shown the effects of α-Bungarotoxin on neuronal nAChR to be highly specific for the α₇-subtype (IC₅₀ = 1.6 nM), but not for the α₃β₄-subtype (IC₅₀ >3 µM).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lopez, M.G., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 14184.Zhang, Z.W., et al. 1994. Neuron 12, 167.Bambrick, L.L., and Gordon, T. 1992. J. Physiol.449, 479.Lin, S.R., and Chang, C.C. 1992. Biochim. Biophys. Acta1159, 255.Motomura, M., et al. 1992. Neurosci. Lett.143, 139.Sorenson, E.M., and Chiappinelli, V.A. 1992. J. Comp. Neurol.323, 1.Ruan, K.H., et al. 1990. Proc. Natl. Acad. Sci. USA87, 6156.

Packaging

1 mg in Plastic ampoule

Physical form

Supplied as an acetate salt

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Sequence

H-Ile-Val-Cys³-His-Thr-Thr-Ala-Thr-Ser-Pro-Ile-Ser-Ala-Val-Thr-Cys¹⁶-Pro-Pro-Gly-Glu-Asn-Leu-Cys²³-Tyr-Arg-Lys-Met-Trp-Cys²⁹-Asp-Ala-Phe-Cys³³-Ser-Ser-Arg-Gly-Lys-Val-Val-Glu-Leu-Gly-Cys⁴⁴-Ala-Ala-Thr-Cys⁴⁸-Pro-Ser-Lys-Lys-Pro-Tyr-Glu-Glu-Val-Thr-Cys⁵⁹-Cys⁶⁰-Ser-Thr-Asp-Lys-Cys⁶⁵-Asn-Pro-His-Pro-Lys-Gln-Arg-Pro-Gly-OH (disulfide bonds: 3 → 23; 16 → 44; 29 → 33; 48 → 59; 60 → 65)

Warning

Toxicity: Harmful (C)