Apoptosis Inhibitor II; NS3694

Biochem/physiol Actions

Target IC₅₀: 50 µM completely blocks TNF-α-induced death in MCF-casp3 cells

Reversible: no

Product does not compete with ATP.

Cell permeable: yes

Primary TargetApoptosome complex formation

General description

A cell-permeable diarylurea compound that specifically prevents the active ~700-kDa apoptosome complex formation triggered by cytochrome c release, thus blocking apoptosome-mediated caspase activation and cell death (50 µM completely blocks TNF-α-induced death in MCF-casp3 cells). Unlike M50054 (Cat. No. 178488), NS3694 exhibits no effect on apoptosome-independent caspase activation and cell death induced by FasL. Inhibits neither cytochrome c release nor the enzymatic activity of caspases.

A cell-permeable diarylurea compound that specifically prevents the formation of the active ~700 kDa apoptosome complex induced by cytochrome c release. Alters cytochrome c and ATP-triggered association between Apaf-1 and caspase-9. Protects against apoptosome-mediated caspase activation and cell death (50 µM completely blocks TNF-α-induced death in MCF-casp3 cells). Unlike M50054 (Cat. No. 178488), NS3694 exhibits no effect on apoptosome-independent caspase activation and cell death induced by Fas ligand (FasL). Does not inhibit cytochrome c release or the activity of caspase-3 and -9.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lademann, U., et al. 2003. Mol. Cell. Biol.23, 7829.

Packaging

10 mg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)