AMPK Inhibitor; Compound C

Code: 171260-5MG D2-231

Biochem/physiol Actions

Reversible: yes

Product competes with ATP.

Cell permeable: yes

Target IC50: 25.1, 148, and 234.6 nM, against KDR/VEGFR2, ...


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€331.30 EACH
€407.50 inc. VAT

Biochem/physiol Actions

Reversible: yes

Product competes with ATP.

Cell permeable: yes

Target IC50: 25.1, 148, and 234.6 nM, against KDR/VEGFR2, ALK2/BMPR-I, AMPK kinase activity, respectively

Primary TargetAMPK

EC100 = 2.5 µM against BMP pathway-dependent dorsoventral development; EC50 = 5 µM against VEGF signaling-dependent intersomitic vessel formation in zebrafish embryo in vivo

General description

A cell-permeable pyrrazolopyrimidine compound that inhibits against KDR/VEGFR2, ALK2/BMPR-I, AMPK kinase activity (IC50 = 25.1, 148, and 234.6 nM, respectively), while exhibiting much reduced or little effect toward ALK5/TGFβR-I, ZAPK, Syk, PKCθ, PKA, or JAK3. Shown to block both BMP pathway-dependent dorsoventral development (EC100 = 2.5 µM) and VEGF signaling-dependent intersomitic vessel formation (EC50 = 5 µM) in zebrafish embryo in vivo. Commonly used in combination with AMPK activators AICAR (Cat. No. 123040) and/or Metformin (Cat. No. 317240) for studying AMPK-dependent cellular events in vitro and physiological responses in animals in vivo. Also available as a 10 mM solution in DMSO (Cat. No. 171261).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hao, J., et al. 2010. ACS Chem.Biol.5, 245.Yu, P.B., et al. 2008. Nat. Chem. Biol4, 33.Kim, E.K., et al. 2004. J. Biol. Chem.279, 19970.Lee, M., et al. 2003. J. Biol. Chem.278, 39653.Inoki, K., et al. 2003. Cell115, 577.Fryer, L.G., 2002. FEBS Lett.531, 189.Zhou, G., et al. 2001. J. Clin. Invest.108, 1167.

Packaging

Packaged under inert gas

1, 5, 10 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)

assay≥95% (HPLC)
colorlight yellow
formsolid
manufacturer/tradenameCalbiochem®
Quality Level100
shipped inambient
solubilitymethanol: 3 mg/mL, 2% acetic acid: 10 mg/mL, 2% HCl: 10 mg/mL, DMSO: 4 mg/mL
storage conditionprotect from light, OK to freeze
storage temp.2-8°C
Cas Number866405-64-3
This product has met the following criteria to qualify for the following awards:



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