Biochem/physiol Actions
Primary Targetchymotrypsin
Product does not compete with ATP.
General description
Specific irreversible inhibitor of serine proteases. Reacts covalently with a serine at the active site. Inhibits chymotrypsin, kallikrein, plasmin, thrombin, trypsin, and related thrombolytic enzymes. Stable, nontoxic alternative to PMSF (Cat. No. 52332) and DFP (Cat. No. 30967). Used at the same molar concentration (0.1-1.0 mM) as PMSF for most applications. Exhibits low inhibitory activity towards proteinase K.
Specific, irreversible inhibitor of serine proteases. Inhibits chymotrypsin, kallikrein, plasmin, thrombin, trypsin, and related thrombolytic enzymes. Stable, nontoxic alternative to PMSF (Cat. No. 52332) and DFP (Cat. No. 30967). A β-secretase inhibitor that inhibits β-amyloid peptide (Aβ) production and enhances amyloid precursor protein (sAPPUα) secretion in several cell lines at millimolar concentrations. Use at the same molar concentrations (0.1-1.0 mM) as PMSF for most applications. Also available as a 50 mM solution in H2O (Cat. No. 508436).
AEBSF, CAS 30827-99-7, is a specific irreversible inhibitor of chymotrypsin, kallikrein, plasmin, thrombin, trypsin, and related thrombolytic enzymes. A nontoxic alternative to PMSF and DFP.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Citron, M., et al. 1996. Neuron 17, 171.Ohkubo, I., et al. 1994. J. Biochem. 116, 1182.Mintz, G.R. 1993. BioPharm. 6, 34.Lawson, W.B., et al. 1982. Folia Haematol.109, 52.Markwardt, F., et al. 1974. Biochem. Pharmacol.23, 2247.Markwardt, F., et al. 1973. Thromb. Res.2, 343.Baker, B.R., and Cory, M. 1971. J. Med. Chem.14, 119.
Packaging
Packaged under inert gas
500 mg in Plastic ampoule
1 g in Glass bottle
50, 100 mg in Glass bottle
Reconstitution
Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 months at 4°C. Slight hydrolysis occurs at alkaline pH (8-9).
Warning
Toxicity: Standard Handling (A)
This product has met the following criteria to qualify for the following awards: