alpha-Amylase from hog pancreas, powder, approx. 50 U/mg

Application

α-Amylase from hog pancreas has been used:in the in vitro digestion system to study the hydrolysis of pea globulinsin vitro α-amylase inhibition studyto prepare digestive juice (salivary juice) for the in vitro model of the human digestion system

Biochem/physiol Actions

α-Amylase is a starch hydrolase, for glucose production, important for energy acquisition. It catalyzes the hydrolysis of α-1,4-glycosidic linkages in starch to form glucose, maltose, and maltotriose units. In mammals, α-amylase regulates the sugar assimilation by reducing the glucose uptake by Na⁺/glucose cotransporter 1 (SGLT1) and stimulating glycoprotein N-glycans mediated starch digestion. α-Amylase inhibitors are associated with the treatment of type 2 diabetes mellitus. α-Amylases are widely known industrial enzymes used in food, detergent, textile, fermentation, and pharmaceutical industries.

General description

Pancreatic α-amylase is produced by the pancreatic acinar cells and released into the duodenum. It is a major molecule of pancreatic fluid.

Other Notes

Optimum pH and temperature 6.9 and 53 °C, respectively; calcium is required for activity and also prevents degradation by trypsin; Characterization.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 µmol maltose per minute at pH 6.9 and 25°C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate)