Analysis Note

Salmonella spp. produce transparent green to blue-green colonies, with or without black centers, or may appear completely black due to H₂S production. Shigella spp. produce green, transparent moist colonies. Lactose, Sucrose or Salicin fermenting gram-negative bacteria may be inhibited or produce red to salmon colored colonies with or without precipitation zone.

Application

GranuCult^® prime Hektoen Enteric Agar is used to isolate and differentiate Salmonella spp. and Shigella spp. from food and other samples.

Features and Benefits

Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment Excellent wettability, solubility, and free flowing properties Convenient, with minimal component separation and clumping, even under warm or humid conditions High batch-to- batch reproducibility Prolonged shelf life of up to five years High number of test strains exceeding all regulatory demands Granulation technology allowing many supplements to be included, with no need to add these separately

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

GranuCult^® prime Hektoen Enteric Agar is a moderately to highly selective media for isolation and differentiation of Salmonella and Shigella species from food and clinical sources. This culture medium provides good colonial differentiation and inhibits some coliforms and other non-lactose-fermenting bacteria due to the presence of bile salts which suppresses the growth of accompanying bacteria. Shigella species overcomes the inhibitory effect of the bile salts due to the presence of relatively large amounts of peptone and carbohydrates. The media has two indicator dyes - bromothymol blue and acidic fuchsin which enables differentiation between lactose fermenters and non-lactose fermenters. Non lactose fermenters (Shigella and Salmonella) appear as bluish-green colonies whereas the lactose fermenters appear as yellowish to red colour colonies. Salmonella is differentiated from Shigella due to its ability to produce H₂S, which reacts with thiosulfate or ferric ions to produce black colour colonies

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

500 g in Plastic bottle