Analysis Note

ControlJurkat cell lysate

Application

Research Sub CategoryHistones

Anti-PRMT1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of PRMT1 & has been validated in WB.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The methylation of arginine is mediated by the Protein Arginine Methyl Transferase (PRMT) family of enzymes. These enzymes transfer the methyl group from S-adenosyl-Lmethionine to the guanidino nitrogen atoms of an arginine residue. Arginine methylation was found to be an important modification in signal transduction, transcription, RNA transport, and splicing. PRMT1, also known as HRMT1L2 and IR1B4, was isolated through its interaction with BTG1 and TIS21, proteins that are important in cell quiescence. PRMT1 is a 361 amino acid protein; its splicing variants differ at the N-terminus, giving rise to three isoforms of 343, 361, and 347 amino acids (variants 1, 2, and 3 respectively). Proteins involved in the metabolism of RNA are substrates for PRMT1, among them Sam68.

Immunogen

Peptide corresponding to residues 298-318 of human protein arginine methyltransferase 1.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A purified

0.1M Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%

Format: Purified

Quality

routinely evaluated by immunoblot in lysates from Jurkat and 3T3/A31, NIH 3T3, HeLa, and mouse ES cells

Specificity

PRMT1 doublet

Storage and Stability

2 years at -20°C

Target description

42-44 kDa