Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results. The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Anaplasma phagocytophilum is a Gram-negative bacterium of the Anaplasma genus that is responsible for causing human anaplasmosis, formerly known as human granulocytic ehrlichiosis. It can also cause disease in dogs, ruminants, horses and sheep. It is an obligate

intracellular pathogen of 0.2-2µm in diameter with a circular genome of approximately 1.48 Mb. It is unable to synthesise peptidoglycan or lipopolysaccharide, relying on membrane cholesterol to maintain physical integrity and leaving the organism extremely vulnerable to stress.

A. phagocytophilum is usually transmitted through tick bites and the majority of cases occur between April and September. The bacterium specifically attaches to neutrophils and persist intracellularly within vacuoles. It produces a number of pathogenic factors that aid virulence

including specific adhesins for neutrophils and virulence factors that inhibit phagosomelysozome fusion and the production of reactive oxygen species that would normally kill the bacterium. The bacterium also inhibits programmed cell death of the neutrophil (apoptosis) and induces expression of interleukin-8, which causes neutrophil chemotaxis, thereby increasing the spread of the bacterium throughout the host organism.

The Primerdesign genesig Kit for Anaplasma phagocytophilum (A.phagocytophilum) genomes is designed for the in vitro quantification of A.phagocytophilum genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design.

• Exceptional value for money
• Rapid detection of all clinically relevant subtypes
• Positive copy number standard curve for quantification
• Highly specific detection profile
• High priming efficiency
• Broad dynamic detection range (>6 logs)
• Sensitive to 100 copies of target
• Accurate controls to confirm findings
• genesig® kits are sold for research use only and are not licensed for diagnostic procedures
• Easy kit contents: target primer/probe mix; lyophilised oasig MasterMix; lyophilised oasig MasterMix resuspension buffer; positive control template; internal extraction control DNA/RNA; water; 54x genesig q16 reaction tubes
• Standard kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); RNAse/DNAse free water
• Advanced kit contents: primer and probe mix (150 reactions); reverse transcription, target specific primers (RNA genome viruses only); copy number standard curve (sufficient for multiple standard curves); internal extraction control - read through VIC channel (150 tests); endogenous control (150 tests); RNAse/DNAse free water