This is a selective differential medium for the isolation of Escherichia coli O157:H7. It differs from other MacConkey medium in that lactose has been replaced by sorbitol. As Escherichia coli O157:H7 does not ferment sorbitol it produces pale translucent colonies whereas most other strains of Escherichia coli are sorbitol positive and produce pink colonies. The medium is made more selective by the addition of the antimicrobial cefixime and potassium tellurite.
- Storage temperature: 2 to 8°C
- Appearance: firm gel
- Colour: pale red with a violet tinge
- Recommended incubation: aerobically at 37°C for 16 to 24 hours
- pH: 7.1 ± 0.2
- Plate size: 90mm
 
                        
                            
                                
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