Tryptone soya agar is used for a wide range of applications, including culture storage, enumeration of cells, isolation of pure cultures, or simply general culture. It has been found to be useful in cosmetic testing, water and wastewater applications. Tryptone soya agar may be used to determine X and V factor requirements of Haemophilus species; sterility testing; and environmental monitoring within pharmaceutical cleanrooms and sterile facilities. This medium meets the requirements of the Harmonised Harmonised USP/EP/JP and is based on the original formulation described by Leavitt et al. in 1955. Tryptone soya agar is recommended as a reference medium when testing selective media, to measure the degree of inhibition. In environmental monitoring applications, it is common for plates to be incubated at 30 to 35°C for bacterial colonies and 20 to 25°C for mould and fungi.
Recommended by the UK Standards for Microbiology Investigations, International Organisation for Standardisation and World Health Organisation and is tested in accordance with ISO 11133:2014. This medium is also included in the Bacteriological Analytical Manual for cosmetics testing.
- Storage temperature: 2 to 8°C
- Appearance: firm gel
- Colour: pale straw
- Recommended incubation: in accordance with the specific investigation and associated methodology performed
- pH: 7.1 ± 0.2
- Plate size: 90mm
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