Meridian Bioscience Air-Dryable Direct DNA qPCR Blood 4x - 1000 Reactions

Air-Dryable™ Direct DNA qPCR and RNA/DNA qPCR Blood mixes are glycerol-free mixes that contain optimised excipients compatible with air and oven drying and they are designed for the direct molecular quantitation of DNA and RNA from whole blood, serum, or plasma. Can also be used wet and/or with purified nucleic acid.

Blood is one of the most common specimens used for laboratory diagnostic testing and it is useful for evaluating the function of vital organs (kidneys, liver, thyroid, and heart) and for diagnosing diseases such as bacterial and viral infections, cancer, cardiovascular disease, and metabolic disorders such as diabetes. However, whole blood specimens, serum, and plasma contain a number of inherent PCR inhibitors including immunoglobulin G, haemoglobin, lactoferrin, and leukocyte DNA. In addition, PCR inhibitors can be found in the anticoagulants used to stabilize blood samples (e.g. EDTA, citrate, or heparin). Traditional methods have relied on removing these inhibitors by DNA or RNA extraction prior to testing, however, these methods are problematic, can cause sample loss, and are not 100% effective at removing all the inhibitors.

Air-Dryable™ DNA qPCR Blood is a 4x qPCR master mix for the development of DNA blood tests, enabling highly sensitive detection of target DNA from whole blood, plasma, and serum samples. Air-Dryable™ RNA/DNA qPCR Blood is a 4x qPCR master mix and can be used for the development of both DNA and RNA blood tests from whole blood, plasma, and serum samples. These mixes have been formulated specifically to overcome the inhibitors found in blood samples – no further optimisation is required aside from the addition of primers and probes. Furthermore, these mixes can be used in a wet format or oven dried to create ambient-temperature stable whole blood tests, plasma tests or serum tests.

• Create ultra-sensitive multiplex direct blood qPCR tests using whole blood, serum or plasma
• Simplify the development of quantitative PCR blood tests, by removing the nucleic acid extraction step and shortening the sample-to-result workflows
• Validated for detection of blood pathogens and liquid biopsy applications
• Can be used wet or air-dried for developing ambient-temperature stable assays