Application

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected AMPA receptor (GluR1-4) in 10 µg of human brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected AMPA receptor (GluR1-4) in 10 µg of transfected human GluA1 HEK293, transfected rat GluA1 HEK293, transfected rat GluA2 HEK293, transfected rat GluA3 HEK293, and Mock transfected cell lysate.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected AMPA receptor (GluR1-4) in human cerebral cortex, human thalamus, rat cerebellum, and rat cerebral cortex tissue.
Immunocytochemistry Analysis: A representative lot detected AMPA receptor (GluR1-4) in Hela cells transfected with GluA1, GluA2, GluA3 and GluA4 (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
Immunofluorescence Analysis: A representative lot detected AMPA receptor (GluR1-4) in rodent, primate and human brain sections (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
Electron Microscopy Analysis: A representative lot detected AMPA receptor (GluR1-4) in rat hippocampus (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
ELISA Analysis: A representative lot detected AMPA receptor (GluR1-4) in TrpE fusion proteins (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).

Anti-pan-AMPA receptor (GluR1-4), clone 2D8 Antibody is an antibody against AMPA receptor for use in Western Blotting, Immunohistochemistry and Immunocytochemistry, Enzyme Immunoassay (ELISA), Electron Microscopy and Immunofluorescence.

General description

Glutamate receptors are synaptic receptors located primarily on the membranes of neuronal cells. Upon ligand stimulation, ionotropic glutamate receptors (iGluRs) form non-selective cation channels, while metabotropic glutamate receptors (mGluRs) indirectly activate ion channels via G protein-mediated signaling. AMPA receptors are iGluRs and are also known as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors, AMPARs, or quisqualate receptors. AMPARs are composed of four types of subunits, GluR1/GluA1 (UniProt P42261) is encoded by GRIA1, GluR2/GluA2 (UniProt P42262) by GRIA2, GluR3/GluA3 (UniProt P42263) by GRIA3, and GluR4/GluA4 (UniProt P48058) by GRIA4 gene in human. AMPARs are tetrameric in nature, consisting of two sets of homodimers, most often a set of GluR2 dimer is coupled with a set of GluR1, GluR3 or GluR4 homodimer. Cat. No. Anti-pan-AMPA receptor (GluR1-4), clone 2D8, reacts with all four AMPA receptor subunits.

Immunogen

Fusion protein coding for the extracellular domain of AMPA Glutamate receptor 1-4 corresponding to human pan-AMPA receptor (GluR1-4).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected AMPA receptor (GluR1-4) in 10 µg of rat brain tissue lysate.

Target description

~85 kDa observed. Uncharacterized band(s) may appear in some lysates.