Application

Research CategoryEpigenetics & Nuclear Function

Anti-TAF-15, clone 12F11, Cat. No. MABE1918, is a highly specific rat monoclonal antibody that targets TATA-binding protein-associated factor 2N and has been tested for use in ELISA and Western Blotting.

Western Blotting Analysis: A representative lot detected TAF-15 in Western Blotting applications (Suarez-Calvet, M., et. al. (2016). Acta Neuropathol. 131(4):587-604).

ELISA Analysis: A representative lot detected TAF-15 in ELISA applications (Suarez-Calvet, M., et. al. (2016). Acta Neuropathol. 131(4):587-604).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

TATA-binding protein-associated factor 2N (UniProt: Q92804; also known as 68 kDa TATA-binding protein-associated factor, TAF(II)68, TAFII68, RNA-binding protein 56, TAF15) is encoded by the TAF15 (also known as RBP56, TAF2N) gene (Gene ID: 8148) in human. TAF15 is a ubiquitously distributed protein that is found in all fetal and adult tissues. It is a RNA and ssDNA-binding protein that plays specific roles during transcription initiation at distinct promoters and can enter the preinitiation complex together with the RNA polymerase II (Pol II). TAF15 shuttles between the nucleus and the cytoplasm. It can be dimethylated by protein arginine N-methyltransferase 1 (PRMT1) at Arg206 to asymmetric dimethylarginine. This methylation favors its nuclear localization and it also considered to be essential for its gene regulatory function. TAF15 contains an N-terminal activation domain, an RNA recognition motif (RRM) and many Arg-Gly-Gly (RGG) repeats at its C-terminal end. Its N-terminus serves as an essential transforming domain in the fusion oncoprotein created by chromosomal translocation in certain human chondrosarcomas. Depletion of TAF15 has a growth-inhibitory effect and results in increased apoptosis. (Ref.: Ballarino, M et al. (2013). Oncogene 32(39); 4646-4655; Jobert, L., et al. (2008). Exp. Cell res. 315(7);1273-1286).

Immunogen

Ovalbumin-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human TATA-binding protein-associated factor 2N (TAF15).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified rat monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TAF-15 in HEK293 cell lysate.

Specificity

Clone 12F11 is a rat monoclonal antibody that detects human TATA-binding protein-associated factor 2N (TAF15). It targets an epitope with in 20 amino acids from the C-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~68 kDa observed; 61.83 kDa calculated. Uncharacterized bands may be observed in some lysate(s).