Application

Analytes: Aldheyde dehydrogenase 1 Family, Member A1 (ALDH1A1), Carbonic Anhydrase IX (CA9), CD44, Epithelial cell adhesion molecule (EpCAM), Hepsin, Kallikrein-6, Mesothelin, Midkine, Neural cell adhesion Molecule L1 (L1CAM), Transglutaminase 2 (TGM2) Recommended Sample Type: Human serum, plasma or tissue/cell/tumor culture supernatant Recommended Sample Dilution: 25 µL per well of 1:5 diluted serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay Assay Run Time: Overnight (16-18 hours) at 2-8°C NOTE: min DC for CA9, Midkine, Kallikrein 6 and EpCAM are in pg/mL while the rest of the analyte min DC are in ng/mL Research Category: Cancer Research Subcategory: Immune Response, Alzheimer′s Disease, Inflammation, Oncology, Tumor Markers

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Circulating cancer biomarkers give insight beyond intracellular oncogenesis and into how the body responds to the presence of a tumor, the dysregulation of homeostasis, and the relationship between a tumor and its environment. Multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology. MILLIPLEX^® Human Circulating Cancer Biomarker Panel 4 is a 10-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum and plasma samples: Aldheyde dehydrogenase 1 Family, Member A1 (ALDH1A1), Carbonic Anhydrase IX (CA9), CD44, Epithelial cell adhesion molecule (EpCAM), Hepsin, Kallikrein-6, Mesothelin, Midkine, Neural cell adhesion Molecule L1 (L1CAM), and Transglutaminase 2 (TGM2). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Circulating Cancer

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Please contact Technical Service for linearity of dilution.